IMAGE

Fig. 5

ID

ZDB-IMAGE-210726-59

Genes

Antibodies

Ab2-kdrl

Publication

Kempers et al., 2021 - The endosomal RIN2/Rab5C machinery prevents VEGFR2 degradation to control gene expression and tip cell identity during angiogenesis

All Figures

Figures for Kempers et al., 2021

Image

Figure Caption

Fig. 5

Rab5C controls Vegf and Notch signaling in vivo. a Western blot analysis of zebrafish lysates probed for Vegfr2 with α-actin as a loading control. A representative blot from n = 3 is shown. b Quantification of Western blots showing relative Vegfr2 levels (normalized to α-actin). Levels in embryos injected with Control MO were set to 1. Results are means + SEM (n = 3), 10 embryos were used per condition per experiment. AU, arbitrary units. c Inverted maximum projections of confocal z-stacks acquired by time-lapse microscopy in Tg(fli1a:lifeact-EGFP)^mu240 zebrafish embryos injected with Control MO (left) or rab5c MO (right). Scale bar, 10 μm. DA, dorsal aorta. dTg(TP1:Venus-PEST);(kdrl-mCherry)^s896 zebrafish embryos demonstrating activation of Notch signaling in embryos injected with Control MOs (top), which is reduced in embryos injected with rab5c MOs (bottom). Scale bar, 20 μm

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Angiogenesis