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Figure 1—figure supplement 4.

ID: ZDB-IMAGE-210726-40
Publication: Isles et al., 2021 - Pioneer neutrophils release chromatin within in vivo swarms
All Figures: Figures for Isles et al., 2021

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Figure Caption

Figure 1—figure supplement 4. Expression of LTB4 signalling components in zebrafish neutrophils.

(A) Single-cell gene expression profiles of LTB4 signalling components expressed in the zebrafish neutrophil lineage, extracted from the Sanger BASiCz zebrafish blood atlas. Circles represent individual cells colour coded where red is high expression and yellow is no expression. (B) Genotyping example of successful CRISPR-induced indels by high-resolution melt analysis for blt1 and lta4h sgRNA injected larvae. Wild-type curves (red) from three representative control tyrosinase larvae and shifted, irregular melt curves (green) corresponding to mosaic heteroduplex PCR fragments formed as a result of CRISPR/Cas9 mutations.

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