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Figure 2

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Publication: Andreana et al., 2021 - Toward Quantitative in vivo Label-Free Tracking of Lipid Distribution in a Zebrafish Cancer Model
All Figures: Figures for Andreana et al., 2021

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Figure 2

In vivo hyperspectral coherent anti-Stokes Raman scattering (CARS) images. (A) CARS image of healthy zebrafish larva at 120 hpf at 2,845 cm⁻¹, field of view (FOV) is 80 × 80 μm². (B) High-resolution CARS image of the green dashed square in (A) showing contrast coming from the lipid content of the muscle cells, FOV is 30 × 30 μm². (C) Quadratic power dependency of the CARS signal of the white dashed rectangle area in (A) respect to the pump power intensity. R-squared of the quadratic fit is 0.9889. (D) Spectral information provided by hyperspectral CARS allowing discrimination between the resonant signal of lipids and 1% agarose gel.

Acknowledgments

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