IMAGE

FIGURE 3

ID: ZDB-IMAGE-210718-36
Publication: Hsiao et al., 2021 - The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects
All Figures: Figures for Hsiao et al., 2021

Image

Figure Caption

FIGURE 3

Developmental timing-specific analysis for the cell proliferation and cell cycle in developing larvae. The larvae of control and FD groups at 26, 48, and 72 hpf were subjected to immunostaining with anti-PH3 antibodies (M-phase marker) (A), as well as TUNEL assay for apoptosis (B). The number of PH3+ cells and apoptotic cells within eye area were quantified by counting the cells with fluorescent signal using the on-line software Image J (C). Larvae at 26 hpf (D) and 72 hpf (E) were dispersed, stained with propidium iodide (PI) and analyzed for cell cycle with flow-cytometry and quantified. White arrows indicate the signals of PH3-positive cells; yellow arrows represent the signals of apoptosis; red dotted lines circle the eye area. Statistical data are shown in mean ± SEM. **p-value < 0.01; *p-value < 0.05.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol