FIGURE 2

FIGURE 2

Morphological and functional characterization on the eyes of FD larvae. (A,B) Control and FD larvae at indicated stages with/without folate supplementation were imaged laterally for the head region and quantified for the eye area (dotted circle) with Image J (An on-line software for scientific image analysis.). The larvae in folate supplemented groups were exposed to the indicated folate adducts at 25 hpf and until observation. (C) The diameter of lens was estimated from the images of H&E stained cryo-sections prepared from the larvae at 5 dpf. (D) The experimental flow of optomotor response analysis (OMR). (E) Control and FD larvae with/without folate supplementation were analyzed for OMR at 7 dpf individually and quantified. WT, wild-type; C (control), heat-shocked wild-type larvae; M, FD transgenic larvae with mild FD. S, FD transgenic larvae with severe FD. L, length of lens; ON, optic nerve; RPE, retinal pigmented epithelium; IS/OS, inner segment/outer segment junction; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; FA, folic acid (1mM); 5-CHO THF, 5-formyltetrahydrofolate (0.5 mM); 5-CH₃ THF, 5-methyltetrahydrofolate (1 mM). Statistical data are shown in mean ± SEM. ***p-value < 0.001; **p-value < 0.01; *p-value < 0.05.