ZFIN Image: Afonin et al., 2021, FIGURE 8

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Figure Caption

FIGURE 8

Cytotoxicity evaluation of cWFW and its fluorescent analogues by fluorescence microscopy. (A) First, at higher magnification (63× objective), the cells were imaged to monitor cell entry of the peptides; HeLa cells with 20 µM peptide imaging after 1 h of incubation are shown for illustration. (B) After imaging (2.5 h after peptide addition), Hoechst/PI co-staining is applied and imaged at 20× magnification, exemplified here by experiments with HEK293 cells; (C) Total/dead cell counting results for all peptides at 20 µM concentration co-incubated with both cell types.

Acknowledgments

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