Fig. 6

Fig. 6

The Hedgehog signalling pathway is regulated by miR500A. (A‐C) Quantification of the mRNA levels of PTCH1 (A), GLI3 (B) and CUL3 (C) by real‐time RT‐qPCR in TERT overexpression conditions. (D) Luciferase activity following cotransfection with pre‐miR500A, in combination with the indicated PTCH1 3′UTR constructs in cells. (E, F) Specific inhibition of PTCH1 by siRNA in pBABE‐SAOS 2 cells. (E) Quantification of PTCH1 level and (F) its effect on the in vivo invasive capacity. (G, H). Correlations between TERT vs miR500A, and miR500A vs PTCH1 expression are shown in invasive STAC (G) and BLCA (H). Spearman's correlation (G, H) reveals a statistically significant positive correlation between TERT and miR500A, and a statistically significant negative correlation between miR500A and PTCH1. Each bar represents the mean ± SEM from triplicate samples, and graphs are the mean of three (N = 3) independent experiments (A–E). Histogram represents the accumulative value of the invasion percentage of the number of larvae stated in the figure for each treatment (F). ns, not significant; *P < 0.05; ***P < 0.001 according to Student's t‐test (A–C, E) and ANOVA followed by Bonferroni's multiple comparison test (D) and Fisher's exact test (F).