FIGURE 4

FIGURE 4

Relative abundance of bacterial genera affected by infection with V. anguillarum. Analysis of the bacterial microbiota was performed in Tg(Bacmpx:GFP)ⁱ¹¹⁴ zebrafish larvae at 8 days post fertilization (dpf), corresponding to 3 days post V. anguillarum infection. Control group: non-inoculated conventionally raised larvae (CONV-R). Dh97 and Yl242 groups: CONV-R larvae inoculated by immersion with 5 × 10⁶ CFU/mL of D. hansenii 97 or Y. lipolytica 242, respectively, at 4 dpf. Va group: CONV-R larvae challenged at 5 dpf by immersion with 10⁷ CFU/mL of V. anguillarum. Dh97 + Va and Yl242 + Va groups: CONV-R larvae inoculated by immersion with both yeasts at 4 dpf and challenged with V. anguillarum at 5 dpf. Significant differences between groups and the control group are marked with black asterisks. Green asterisks indicated significant differences between Dh97 + Va or Yl242 + Va with Va group (p < 0.05, Kruskal–Wallis followed by Dunn’s test).