ZFIN Image: Vargas et al., 2021, FIGURE 2

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Figure Caption

FIGURE 2

Bacterial diversity analyses of Tg(Bacmpx:GFP)ⁱ¹¹⁴ larvae inoculated with yeasts and/or V. anguillarum. (A) Alpha diversity indexes Chao1, Shannon, and Simpson. (B) Principal coordinate analysis (PCoA) based on weighted Unifrac distance matrix plot. Analysis of the bacterial microbiota was performed in Tg(Bacmpx:GFP)ⁱ¹¹⁴ zebrafish larvae at 8 days post fertilization (dpf), corresponding to 3 days post V. anguillarum infection. Control group: non-inoculated conventionally raised larvae (CONV-R). Dh97 and Yl242 groups: CONV-R larvae inoculated by immersion with 5 × 10⁶ CFU/mL of D. hansenii 97 or Y. lipolytica 242, respectively, at 4 dpf. Va group: CONV-R larvae challenged at 5 dpf by immersion with 10⁷ CFU/mL of V. anguillarum. Dh97 + Va and Yl242 + Va groups: CONV-R larvae inoculated by immersion with both yeasts at 4 dpf and challenged with V. anguillarum at 5 dpf. ^∗p < 0.05.

Acknowledgments

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