Fig. 2

Fig. 2 Loss of ppargc1a Results in Ciliogenesis Defects

(A) Schematic indicating location of MCCs in the proximal portion and monociliated cells in the distal portion of the zebrafish pronephros at 24 hpf.

(B) WT (top) and ppargc1a^{sa13186/sa13186} mutant (bottom) zebrafish at 24 hpf stained via WISH for odf3b to mark MCCs. Scale bar, 90 μm.

(C) Graph representing the number of MCCs present in WT and ppargc1a^{sa13186/sa13186} zebrafish at 24 hpf.

(D) Fluorescent in situ hybridization for MCC marker (odf3b, green) and transporter cell marker (trpm7, red) in WT and ppargc1a morphant zebrafish at 24 hpf. Scale bar, 10 μm.

(E and F) Graphs representing the percentage of odf3b+ (E) or trpm7+ (F) cells in WT (black) and ppargc1a morphants (white) pronephros between somites 9 and 11.

(G) Twenty-eight hours post-fertilization WT (top), ppargc1a MO-injected (middle), and ppargc1a^{sa13186/sa13186} (bottom) zebrafish stained via whole-mount immunofluorescence for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI in the proximal pronephros. Scale bar, 10 μm.

(H) Cilia length in micrometers for the proximal pronephros.

(I) Graph of the percentage of ciliated basal bodies/total basal bodies per 100 μm in the proximal pronephros.

(J) Twenty-eight hours post-fertilization WT (top) and ppargc1a MO-injected (middle) and ppargc1a^{sa13186/sa13186} (bottom) zebrafish stained via whole-mount immunofluorescence for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI in the distal pronephros. Scale bar, 10 μm.

(K) Cilia length in micrometers for the distal (right) pronephros.

(L) Graph of the percentage of ciliated basal bodies/total basal bodies per 100 μm in the distal pronephros.

(M and N) Fluorescent intensity plots of WT (black) and ppargc1a MO-injected (red) zebrafish in proximal (M) and distal (N) pronephros. Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 (one-way ANOVA or t test).