Figure 1.

Figure 1.

fmn2b mRNA is maternally deposited and enriched in the zebrafish nervous system. Amino acid sequence alignments were obtained using EMBL Clustal Omega for human Fmn2 (hFmn2), mouse Fmn2 (mFmn2), zebrafish Fmn2b (zFmn2), and chicken Fmn2 (gFmn2) to compare conservation of sequence across vertebrate species. Percent identity matrices are shown for comparison of full length Fmn2 and the functional domains of Fmn2 across species. A, Full-length zFmn2 showed 53.82%, 53.68%, and 54.16% sequence similarity for human, mouse, and chicken Fmn2, respectively. A’, On comparing the functional domains of Fmn2, namely FH1, FH2, and FSI domain, we found 68.34%, 70.43%, and 70.41% sequence similarity with human, mouse, and chicken Fmn2, respectively. B–J, Representative images of whole-mount mRNA in situ hybridization showing the fmn2b mRNA expression pattern in the developing zebrafish embryo. fmn2b mRNA can be found in (B) one cell stage and (C) 3-hpf embryos suggesting maternal deposition. The mRNA expression is negligible during (D) 6 hpf, (E) bud stage, (F) 10-somite, and (G) 15-somite embryos. fmn2b mRNA expression is regained in (H) 24-hpf embryos and continues to be expressed until 96 hpf (I–J’). hpf, hours post fertilization.