Figure 8

Figure 8

Rgs4 function, Akt and Erk activities that mediate Rgs4-dependent motoneurons outgrowth are all required within motoneurons. (A) Representative maximum projections of z-stack optical sections of CaP and MiP at 30 hpf following injection of different constructs allowing the expression of mcherry in WT and MZrgs4, Rgs4-P2A-mcherry in MZrgs4, caAkt-P2A-mcherry in MZrgs4 and caErk-P2A-mcherry in MZrgs4 specifically in motoneurons under the control of hb9 promoter. (B) Quantification of individual CaP motor axons length following injection of hb9:mcherry in WT (average of 84.33 ± 3.48 μm, 6 CaP, n = 4) and MZrgs4 (30.57 ± 4.41 μm, 7 CaP, n = 5), injection of hb9:rgs4-P2A-mcherry in MZrgs4 (average of 76 ± 4.91 μm, 4 CaP, n = 4), injection of hb9:caAkt-P2A-mcherry in MZrgs4 (average of 89.67 ± 8.24 μm, 6 CaP, n = 6) and injection of hb9:caErk-P2A-mcherry in MZrgs4 (average of 87.67 ± 5.73 μm, 6 CaP, n = 6). (C) Quantification of individual MiP motor axons length following injection of hb9:mcherry in WT (average of 54.20 ± 0.66 μm, 5 MiP, n = 4) and MZrgs4 (20 ± 3.23 μm, 6 MiP, n = 5), injection of hb9:rgs4-P2A-mcherry in MZrgs4 (average of 52 ± 0.82 μm, 4 MiP, n = 4), injection of hb9:caAkt-P2A-mcherry in MZrgs4 (average of 58 ± 1.92 μm, 5 MiP, n = 5) and injection of hb9:caErk-P2A-mcherry in MZrgs4 (average of 55.71 ± 2.05 μm, 7 MiP, n = 7). ns, non significant.