Figure 6

Figure 6

Akt and Erk activities mediate Rgs4-dependent PLLg development and motoneurons branching. (A) Quantification of the number of neurons in the PLLg in WT (average of 55.83 ± 1.02, n = 18), WT + caAkt (average of 51.18 ± 0.65, n = 11), WT + caErk (average of 49.90 ± 1.35, n = 10), MZrgs4 (average of 28.35 ± 1.28, n = 20), MZrgs4 + caAkt (average of 55 ± 1.36, n = 22) and MZrgs4 + caErk (average of 45.08 ± 0.64, n = 12). (B–G) Z projections of whole-mount immunostaining for Znp1 labeling the ramifications of motoneurons in WT (B), MZrgs4 (C) WT + caAkt (D), MZrgs4 + caAkt (E), WT + caErk (F) and MZrgs4 + caErk (G) at 48 hpf. Scale bar = 20 μm. (H) Quantification of the number of ramifications per motoneuron in WT (average of 21.08 ± 1.10, 40 motoneurons, n = 12), WT + caAkt (average of 15.10 ± 0.87, 30 motoneurons, n = 10), WT + caErk (average of 14.27 ± 0.83, 33 motoneurons, n = 11), MZrgs4 (average of 9.28 ± 0.66, 46 motoneurons, n = 14), MZrgs4 + caAkt (average of 20.20 ± 1.28, 32 motoneurons, n = 10) and MZrgs4 + caErk (average of 19.83 ± 1.00, 40 motoneurons, n = 12) at 48 hpf.