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Figure 3 OD29 antagonizes VEGF-induced pERK MAP kinase in HUVECs. (A–C) Western blot analysis of dose-dependent effects of small molecule VEGFR kinase inhibitor Axitinib (A), OD16 (B), OD29 (C), or vehicle control (DMSO) on VEGF (40 ng/mL)-induced VEGFR and ERK MAP kinase phosphorylation in HUVECs. (D) Quantification of the Western blot analysis of VEGF-induced p-ERK MAP kinase. Tubulin was used as a loading control. The results from three biologically independent Western blot experiments were integrated. The results are expressed as mean ± SD. (E,F) RT-qPCR analysis of the effects of Axitinib, OD16, OD29 (0.5 µM), or vehicle control (DMSO) on VEGF (40 ng/mL)-induced DLL4 (E) and NR4 A (F) mRNA expression in HUVECs. Results from three biologically independent experiments are shown as mean ± SD. * p < 0.05, ** p < 0.005, *** p < 0.001. NS, not significant. Complete Western Blot images of subfigures (A–C) are available in Figure S10.