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Fig. 1 DNA sequence and predicted protein structure of an established stm gene-edited strain. (A) Genome structure and DNA sequence of the target site for the genome editing of stm. DNA sequences around the target site (in red) for CRISPR/Cas9 digestion in WT and stm−/− mutants are indicated. A 14-nucleotide deletion and 1-nucleotide insertion were induced in the target site in the selected mutant. (B) The predicted protein structures of the WT and the stm−/− mutant are indicated. It is expected that a peptide of 98 amino acids in length with an N-terminal 76-amino acid sequence is the same as WT Stm and is produced in the stm−/− mutant. Thus only first high conserved internal repeat (R1) is present in Stm protein produced in the stm−/− mutant.