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Figure 3 Cyp1b1 F0 crispants’ phenotypes (144 hpf). (A–D) One-cell embryos were microinjected with CRISPR/Cas9 ribonucleoprotein complexes targeting cyp1b1 exon 1. Crispants’ morphology was assessed microscopically at 144 hpf. (E,F) Non-injected larvae were used as controls (wt). White arrow: lower jaw underdevelopment. Red arrowhead: altered swim bladder development. Red dotted circle: wildtype ocular periphery is indicated as a reference to show microphthalmia. Yellow arrowhead: pericardial edema. Scale bar in (A,C,E) = 500 µm. Scale bar in (B,D,F) = 250 µm. (G) Proportion of F0 mutant phenotypes. The controls were microinjected with all CRISPR/Cas9 reagents except crRNAs. Asterisks indicate statistical significance compared to the control, p < 0.05 (*). The values correspond to the means ± SEM of three independent experiments (50–80 embryos per group and experiment).