Fig 2

Fig 2 The glycosylation pathway enzyme Dolk regulates the magnitude of the startle response.

(A) Gene structure for dolk with the nonsense mutation from the screen noted (p420). The CRISPR 1 bp deletion allele is also noted (p421). (B) Protein structure for Dolk on the endoplasmic reticulum [48], with the predicted amino acid change from the screen mutation indicated. The deletion in the CRISPR allele causes a frame shift in the sequence that results in a premature stop at amino acid 50. In contrast to siblings (C-G), dolk mutants (H-L) display an exaggerated startle response, with an increased bend and counter bend angle (I,J), resulting in larvae swimming in a “figure eight” (K). Blue bar = latency in G,L. (M-O) Kinematic parameters of the acoustic startle response in dolk siblings and mutants (homozygotes of the screen identified mutation, p420/p420, and transheterozygotes from the screen mutation and CRISPR, p420/p421). Each point represents average of ten trials for an individual fish. n≥6 larvae, **p<0.001, *p = 0.008 (one-way ANOVA with Tukey correction for multiple comparisons).