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FIGURE 10 Whole mount in situ hybridization for the dorsal organizer marker gene goosecoid (gsc) in wt embryos at the shield stage, showing the effects of control (EGFP 100 pg, 35/35 embryos, arrowheads: normal gsc expression domain), Wnt3-EGFP (100 pg, 31/35 embryos, arrowheads: reduction in gsc expression domain), Wnt3C80A EGFP (100 pg, 38/41 embryos, arrowheads: no effect on gsc expression domain), and Wnt3S212A-EGFP (100 pg, 32/34 embryos, arrowheads: no effect on gsc expression domain).
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Full text @ Front Cell Dev Biol