FIGURE 5
Expression profile of the mutants co expressed with PMT-mApple. Co-expression of mutants (Wnt3C80A-EGFP, Wnt3S212A-EGFP, and Wnt3S212AC80A-EGFP) and PMT-mApple in the zebrafish brain at ∼48 hpf. PMT-mApple is a protein located on the inner leaflet of the cell membrane. It is expressed under a 4kb Wnt3 promoter and serves to mark the source cells of Wnt3 expression.