IMAGE

Fig. 5

ID

ZDB-IMAGE-210614-21

Genes

GFP
mCherry

Publication

Karampelias et al., 2021 - Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation

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Figures for Karampelias et al., 2021

Image

Figure Caption

Fig. 5 Folinic acid stimulates β-cell regeneration in juvenile zebrafish.

a–d Single-plane and maximum projection confocal images of pancreata in control (a–a”) or folinic acid-treated (b–b”) 1-month-old juvenile zebrafish on the Tg(ins:H2BGFP);Tg(ins:flag-NTR);Tg(tp1:H2BmCherry) background, following β-cell ablation. Quantification showed an increase in the number of regenerating β-cells in the secondary islets upon treatment with folinic acid (c) as well as an increase in the number of ins:H2BGFP⁺tp1:H2BmCherry⁺ cells (d). The dashed line outlines the pancreas. Arrowheads point to ins:H2BGFP⁺ cells in the secondary islets. Scale bar, 20 μm. n = 8 (control) and n = 9 (folinic acid) biologically independent zebrafish were used for the quantification of this experiment. Data are presented as mean values ± SEM. Unpaired two-tailed Student’s t test was used to assess significance for (c) and (d). c *P = 0.0264; d *P = 0.0319.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.