Figure 3.

Figure 3.

Expression of botulinum toxin in GCs suppresses active avoidance conditioning. A, B, Turning frequency (turns/min) and swimming speed (mm/s) of gSA2AzGFF152B;Tg(UAS:BoTxBLC-GFP) (152B::BoTx) and control sibling fish during free swimming (152B::BoTx; n = 47, control; n = 43). The graph shows the averages and SEs of the data (ns indicates non-significance, Welch’s t test. C, D, Turning frequency and swimming speed of Tg(cbln12:Gal4FF);Tg(UAS:BoTxBLC-GFP) (cbln12::BoTx) fish during free swimming (cbln12::BoTx; n = 39, control; n = 38). The graph shows the averages and SEs of the data (ns indicates non-significance, *p < 0.05, Welch’s t test). E, F, Response to electric shocks in 152B::BoTx (n = 7) and cbln12::BoTx (n = 7) fish. Swimming speed was calculated for 2 s before and after the electric shocks (***p < 0.001, *p < 0.05, Welch’s t test). G, H, Percentages of active avoidance learners for 152B::BoTx (n = 47) and control sibling fish (n = 43; G), and for cbln12::BoTx (n = 39), and control sibling fish (n = 38; H; ***p < 0.001, *p < 0.05, Fisher’s exact test). ns, not significant.