Fig. 7

Fig. 7 (A) Rescue Tan I induced phenotype by overexpression of ezh2 in the zebrafish embryo. Fluorescent images of c-myb GFP+ cells in Tg(c-myb^hyper:GFP) and Tg(hsp70l:ezh2:CG) double transgenic zebrafish embryos, with DMSO or Tan I treatments and with or without a 37 ºC heat shock for 1h, as indicated. dpf (days post-fertilization) (B) Rescue Tan I induced phenotype by knockdown of mmp9 and abcg2 in the zebrafish embryo. Fluorescent images of c-myb GFP+ cells in Tg(c-myb^hyper:GFP) transgenic zebrafish embryos. As indicated, mmp9 and abcg2d morpholino was injected separately or together in DMSO or Tan I treated embryos. (C-E) Overexpression MMP9 in NB4 cells. RT-qPCR analyses of MMP9 mRNA level on the established NB4 cells transduced with an empty vector or MMP9-expressing vector (C). Flow cytometric analyses of Annexin V/7-AAD staining (D) and CD11b expression (E) in control or MMP9-overexpressed NB4 cells; (F-H) Overexpression of ABCG2 in NB4 cells. RT-qPCR analyses of ABCG2 mRNA level on the established NB4 cells transduced with empty vector or ABCG2-expressing vector (F). Flow cytometric analyses of Annexin V/7-AAD staining (G) and CD11b expression (H) in control or ABCG2-overexpressed NB4 cells. Scale bars: 200 µm.