ZFIN Image: Okuda et al., 2021, Figure 6.2

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Figure Caption

Figure 6.2 Ca<sup>2+</sup> signalling is required for rapid Erk activation in ablated ISV ECs.

(A–T’) Ca²⁺ signalling is required for rapid Erk activation. Lateral spinning disc confocal images of ISV endothelial cells (ECs) in 4 days post-fertilisation (dpf) EC-EKC larvae treated with either 1% dimethyl sulfoxide (DMSO) (A–D’, G–H’ ,K–N’, Q–R’), 50 μM Nifedipine (E–F’, I–J’), or 100 μM Amlopidine (O–P’, S–T’). Images (A-B’) and (K-L’) show non-ablated control ISV ECs. Images (C, E, G, I, M, O, Q, S) were taken pre-ablation and images (D, F, H, J, N, P, R, T) were taken 15 min post-ablation (mpa). Images (A-T) show fli1aep:EKC expression, and images (A-T’) show the nuclear fli1aep:EKC intensity. White dotted lines show the wounded site of each larva. ISV: intersegmental vessel. Scale bar: 15 μm.

Acknowledgments

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