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Figure Caption
Figure 2 SMYD3 depletion does not interfere with stemness. (A) mRNA was extracted at different stages of ESC differentiation, and Smyd3 or pluripotency-related gene (Nanog, Klf4, Pou5f1/Oct4) transcript levels were evaluated throughout differentiation by qRT-PCR. Means and SEM from three independent experiments are shown. (B) Nanog, Oct4 and SMYD3 protein levels were analyzed by immunoblot. Data were normalized to GAPDH. Normalized band intensity in immunoblots is reported below signals. Representative image of three independent experiments.
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