ZFIN Image: Basnet et al., 2021, Figure 6

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Figure Caption

Figure 6

Microinjection of 3F2T cells in perivitelline space and subsequent treatment with caffeine. (A) Representative picture showing the ectopic sprouts branching out of SIVP visualized by AP assay in microinjected embryos at 72 hpf embryos (magnification 63×). (B) Percentage of embryos showing negative, mild positive and strong positive responses following microinjection of 3F2T cells, with or without caffeine treatment. In the bar diagram: CT-: negative control; Cells + C100: Cells + caffeine 100 mg/L; Cells + C150: Cells + caffeine 150 mg/L. Statistical analysis was performed with GraphPad Prism software, version 8.3.0 (GraphPad Software, Inc., La Jolla, CA, USA). *** p < 0.001, when compared to the control group.

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