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Figure Caption
Figure 4 Caffeine exposure decreases the number of interconnecting vessels in the SIVP. (A,B) The panels show representative pictures of AP assay performed in control and treated embryos at 72 hpf (magnification 32×), with an enlargement of the SIVP (magnification 63×) in C and D. (E) The bar diagram shows the average number of SIV branches in zebrafish embryos at 72 hpf (three replicates were performed, n = 15 for each group). Statistical analysis was performed with GraphPad Prism software. *** p < 0.001, when compared with the control group.
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