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FIG 2 The cytosolic localization of M. marinum in zebrafish is abundant when the adaptive immune system is not yet developed. Embryo and adult zebrafish infected with M. marinum were analyzed using TEM. (A) Cytosolic M. marinum in zebrafish embryo tissue. (B) Phagosomal M. marinum ?ESX-5 bacteria in adult zebrafish tissue. (A? and B?) Schematic representations of panels A and B, with black dots indicating actin immunogold labeling, orange lines indicating M. marinum, blue lines indicating phagosomal and host membranes, and green lines indicating mitochondria. (C) Quantification of the percentage of cytosolic M. marinum bacteria at embryo day 9 and in the spleen of adult zebrafish at day 11 (see also Fig. S2B in the supplemental material). Error bars indicate standard deviations between 3 different zebrafish embryos and 3 adult fish, and n represents the total number of bacteria counted.