ZFIN Image: Knickmeyer et al., 2021, Figure 5

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Figure Caption

Figure 5 pax2a expression domains are differentially altered in bmp4 induction and Shh inhibition conditions. (A–J) In situ hybridization of pax2a. (A–D) 21 hpf in (A) control and (B) tg(hsp70l:bmp4) embryos after heat shock at 15 hpf; (C) ethanol control and (D) Cyclopamine-treated embryos. (E–J) 42 hpf in (E) control and (F) tg(hsp70l:bmp4) embryos after heat shock at 15 hpf. Part of the pax2a domain extends dorsally (arrows). (G) Ethanol control and (H–J) Cyclopamine-treated embryos with different expression patterns (n = 12); (E’–J’) show a more anterior region in the same embryos as (E–J). All images are maximum intensity projections, DAPI counterstaining, transverse view, scale bars 50 µm.

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Acknowledgments

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