Figure 4

Figure 4

MMP-9 inhibition induces a change in macrophage shape and a transition towards an amoeboid-like migration. (a) Graph compares the macrophage shape distribution in the AGM of MMP-2 and 9 (SB-3CT) -inhibited embryos (MMP inh) to DMSO treated embryos (control) shows an increase in round shape and a decrease in star-like and elongated shapes in MMP inh embryos. N = 10 embryos for control and 15 for MMP inh. Data represent the percentage mean for each shape type out of the total number of macrophages in the AGM ± s.e.m. NS not significant; *p < 0.05; ****p < 0.0001. (b–e) Selected cropped images from Video 3 displays macrophage shape and migration patterns over time. Numbers point to individual macrophages, time code is expressed in hours and minutes. Grey outlines on panel C-E show the shape and position of macrophages from panel (b) (3rd minute). (f) Graph showing the velocity of macrophages in control and MMP-inhibited embryos. Data are represented as a mean ± s.e.m., n = 17 macrophages for control and 14 for MMP inhibitor from 4 different embryos, ****p < 0.0001. (g,h) Tracking plot diagram represents the migration path and distance of macrophages in the AGM in control and MMP-inhibited embryos measured every minute over 60 min. Scale in µm, n = 17 macrophages for control and 14 for MMP inhibitor from 4 different embryos. See also Video 3. Scale bar 30 µm. DA dorsal aorta, PCV posterior cardinal vein.