ZFIN Image: Stevenson et al., 2021, Figure S4.

Image

Figure Caption

Figure S4.

Procollagen processing in giantin KO MC3T3 cells. (A) Immunoblots of cell lysates taken from WT and giantin KO MC3T3 cells. Antibodies used are as indicated. (B) Single-plane widefield images of WT and giantin KO MC3T3 cells immunolabeled for giantin (green) and the cis-Golgi marker GM130 (magenta). The nucleus is stained in DAPI. Scale bar = 10 µm. (C) Immunoblots of a GFP trap of WT and giantin KO MC3T3 cell cultures transiently expressing GFP-COL1A1. Media and lysates were assayed after 24 h of ascorbate treatment, which was conducted 24 h after transfection. The input (I), unbound (U), and bound (B) fraction of each pull-down is shown probed with either SBP or tubulin antibodies as indicated underneath each blot. Black arrows indicate full-length procollagen, and red arrows the N-propeptide.

Acknowledgments

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