IMAGE

Fig. 5

ID

ZDB-IMAGE-210508-20

Genes

trap1

Publication

Laquatra et al., 2021 - HIF1α-dependent induction of the mitochondrial chaperone TRAP1 regulates bioenergetic adaptations to hypoxia

All Figures

Figures for Laquatra et al., 2021

Image

Figure Caption

Fig. 5 TRAP1 is overexpressed in pancreatic tumors and downregulates SDH activity.

a Western blot analysis and protein quantification of TRAP1 in wild-type pancreas and in pancreatic tumors driven by KRAS^G12D expression (1 year post-fertilization, ypf). Values were normalized for citrate synthase. b Analysis of TRAP1 mRNA expression in wild-type pancreas and in pancreatic tumors at 1 ypf. Values were normalized for expression of rpl13 and actin, used as housekeeping genes. The number of animals used in each condition is reported inside column bars. c Colorimetric assay (pink staining) of SDH activity in tissue slides of wild-type pancreas and pancreatic tumors with/without the TRAP1 specific inhibitor compound 5 (100 μM, 30 min pre-incubation). In (a, b), data are reported as average ±SEM of at least four different animals per condition with an unpaired two-tailed Student’s t test; asterisks indicate significant differences (*p < 0.05).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.