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Fig. 4

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ZDB-IMAGE-210508-19
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Figures for Laquatra et al., 2021
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Figure Caption

Fig. 4 a Schematic representation of the TRAP1 promoter region analyzed that encompasses position −5000 bp to +500 bp from the transcription initiation site. b List of the four (I–IV) HRE regions more conserved between human (Homo sapiens, Hs) and Zebrafish (Danio rerio, Dr) TRAP1 promoters. Scoring was calculated using the position-specific matrix. c Alternative hypoxia-dependent regulative motifs found within the TRAP1 promoter region of Homo sapiens and Danio rerio. d, e Analysis of TRAP1 mRNA expression in wild-type Zebrafish embryos (d) and MIA PaCa-2 human pancreatic adenocarcinoma cells (e) exposed to hypoxia for the indicated times. Values were normalized for expression of rpl13 (d) and actin (e) used as housekeeping genes. f Western blot analysis and protein quantification of TRAP1 expression in embryos at 96 hpf following a 48 h treatment with 100 and 150 µM dimethyloxalyglycine (DMOG). g Western blot analysis and protein quantification of TRAP1 expression in embryos at 96 hpf following a 5 h treatment with 1 mM dimethylsuccinate (DMS). Data are reported as average ±SEM of at least three independent experiments with an unpaired two-tailed Student’s t test; asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001).

Acknowledgments
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