Fig. 2 Effect of TBBPA and TiO2 NP on motor behaviors. Zebrafish embryos were exposed to 0.1% DMSO control, 2 μM TBBPA, 0.1 mg/L TiO2 NP and TBPPA/TiO2 NP mixture from 8 to 120 hpf and motor behaviors were evaluated at different developmental stages. (A) Spontaneous movement (expressed as tail bents/min) at 24 hpf (n = 24). (B) Touch response in dechorionated embryos at 48 hpf (n = 24). (C-D) Free swimming activity (expressed as swim speed) at 5 dpf during a 10-min light period followed by a 10-min dark period (n = 24). Values plotted are mean ± SEM and bars sharing the same letter indicate no significant difference at the level of P < 0.05 using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test.
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