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Figure 4 Nutrition influences on adipocyte development and maintenance. (a,b). Zebrafish embryos at 5 dpf were fed for different durations per day as indicated, and adipocyte development was examined. n ? 12. Repeat = 3. * indicates p < 0.05, and ns indicates p > 0.05. (a) Graphical presentation of the age of larvae that developed the first adipocyte in three groups. (b) Graphical presentation of the body length of larvae that developed the first adipocyte in three groups. ns, not significant. (c) 16 dpf larvae were selected and subjected to fasting for 8 days, and then refed for another 4 days. Representative bright field and fluorescent images are shown to orient the larvae with visceral adipose tissues marked by white arrowheads. n ? 5. Repeat = 3.