ZFIN Image: Wright et al., 2021, Fig 5

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Figure Caption

Fig 5 Cxcr4 reduction places the Cxcl12/Cxcr4 signalling axis downstream of miR-206.

(A) Whole body neutrophil counts at 3 dpi of cxcr4b and double (cxcr4b and miR-206) knockdown embryos. (B) Measurement of neutrophil levels following trunk infection with M. marinum in double knockdown embryos. (C) Bacterial burden at 3 dpi in M. marinum-infected double knockdown embryos. (D) Whole body neutrophil counts at 3 dpi in miR-206 knockdown embryos treatment with AMD3100. (E) Measurement of neutrophil recruitment to M. marinum following trunk injection in miR-206 knockdown embryos treatment with AMD3100. (F) Bacterial burden at 3 dpi in miR-206 knockdown embryos treatment with AMD3100. Each data point represents a single measurement, with the mean and SEM shown. For time-lapse imaging, each data point represents the mean of 6 foci of infection from 6 separate embryos. Bacterial burden analysis was performed on 15–25 embryos per treatment. Graphs are representative of 2 biological replicates, except for AMD3100 data, which is a single biological replicate.

Acknowledgments

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