(A) Representative images of infection phenotype at 3 dpi in control and miR-206 knockdown embryos. White arrows indicate bacterial foci. Neutrophils are red (lyzC:dsred) and M. marinum is green (wasabi); co-localisation is indicated by yellow fluorescence. (B) Measurement of whole-body neutrophil fluorescent area at 1 and 3 dpi in miR-206 knockdown embryos. (C) Measurement of neutrophil levels following trunk infection with M. marinum in miR-206 knockdown embryos. (D) Measurement of neutrophil retention at sites of infection following trunk infection with M. marinum in control and miR-206 knockdown embryos. (E) Measurement of neutrophil recruitment to a sterile tail fin wound in miR-206 knockdown embryos. Each data point represents a single measurement, with the mean and SEM shown. For time-lapse imaging, each data point represents the mean of 6 foci of infection from 6 separate embryos. Neutrophil retention was measured by selecting 10 random cells in 3 embryos per group and measuring the time spent at the granuloma. Bacterial burden analysis was performed on 15–20 embryos per treatment. Graphs are representative of 2 biological replicates. * P < 0.05, ** p < 0.01, *** p < 0.001.
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