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Figure 11 Combination compounds enhance the expression level of melanogenic genes. By the method of in situ hybridization on whole-mount zebrafish embryos to investigate the effects of the best drugs combination on the expression of melanogenic genes (tyr, mitfa, kit and dct). Zebrafish larvae were incubated with combination of butin, caffeic acid and luteolin (butin:caffeic acid:luteolin = 7.38:28.30:64.32). In 35 hpf, collecting zebrafish and hybridizing with labeled nucleic acid probes. The color rendering (blue) is means expression of specific genes, the red, purple, black and green arrows indicate the expression sites of tyr, mitfa, kit, and dct, respectively. The scale bars represent 300 μm.