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Figure 7 (A) Calcein vital staining of scales from treated fish and untreated controls. Control scale show normal profile, while scales from treated fish show central resorption lacunae after 3 weeks of treatment (T3, white arrow) (scale bar: 0.1 mm). (B) Biochemical ALP activity assay indicates a downregulation of anabolic activity in the scale after 5 weeks of treatment (T5) (CTR vs. T5, p < 0.001). The value of the control at each experimental point was omitted as it was identical to the initial CTR. (C) Histological ALP assay confirmed the loss of enzymatic activity (white arrows) in the center of scales after 5 weeks of SBM treatment (scale bar: 0.1 mm). Significance values were set at less than p < 0.001 (***).