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Figure 2 Gene expression analysis by RT-PCR for inflammatory marker genes on zebrafish intestine untreated (CTR) or treated with SBM for one (T1), two (T2), three (T3), four (T4), and five weeks (T5). IL-6, IL-10, TGFβ, IL-1β, IL-8, and TNFα resulted to be statistically significantly increased after two weeks (IL-1β: CTR vs. T2, p < 0.001; IL-8: CTR vs. T2, p < 0.001; IL-10: CTR vs. T2, p < 0.001; TGFβ: CTR vs. T2, p < 0.001; IL-6: CTR vs. T2, p < 0.001; TNFα: CTR vs. T2, p < 0.05) and IL-8 and TNFα after three weeks (IL-8: CTR vs. T3, p < 0.05; TNFα: CTR vs. T3, p < 0.01). After four and five weeks of treatment, only IL-8 resulted to be significantly increased (IL-8: CTR vs. T4, p < 0.001 and IL-8: CTR vs. T5, p < 0.001, respectively). Significance values were set at less than p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).