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Figure 7 Cardiovascular parameters measured at the dorsal aorta (DA) of 3 dpf Tg(flk:EGFP) transgenic zebrafish. (A1) Images of the DA captured by fluorescence microscope (20×) (a–e) Vasodilation observed by treatment with DK. (a) PBS (Control); (b) 4 μM of DK; (c) 13 μM of DK; (d) 40 μM of DK; (e) 134 μM of DK. (A2) Evaluation of DA vessel diameter. (B) Arterial pulse (beats per minute); (C) Mean blood flow velocity (μM/s); (D) Blood flow (nL/s) were measured from n = 6 larvae per group. The yellow box represented the area used for evaluation. Results are expressed as the mean ± standard deviation (S.D.). * p < 0.05, ** p < 0.01. ## p < 0.01. *** p < 0.001 significantly different compared with the control group. dpf, days post-fertilization; ns, not significant; -: no sample treatment (control)