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Figure 5 Influence of a specific antagonist on [Ca2+]cytol levels in EA.hy926 cells treated with DK. (A) Traces and (B) box plots indicating [Ca2+]cytol levels in response to treatment with DK (134 μM) and antagonist. (C) Effect of DK on NO production in EA.hy926 cells pretreated with atropine. The NO levels were detected by adding 10 μM of 4 amino-5-methylamino-2′, 7′-difluorescein diacetate (DAF-FM-DA). Results are expressed as the mean ± standard deviation (SD) of three independent experiments, *** p < 0.001 significantly different compared with the control group; ### p < 0.01 significantly different compared with DK only group. DK, Dieckol; ns, not significant; AUC, area under the curve; [Ca2+]cytol, calcium level in the cytosol; NO, nitric oxide; -: no sample treatment (control)