Figure 3 Quantification of [Ca2+]cytol levels following treatment with different ECE and DK concentable 926. cells. In the absence of [Ca2+]cytol using the Fluo-4 calcium indicator, (A) traces and (B) box plot representation of [Ca2+]cytol levels under ECE treatments (3, 10, 30, and 100 µg/mL); The absence of [Ca2+]cytol levels in cell (C) traces and (D) box plot representation of [Ca2+]cytol levels in response to the addition of DK (0, 4, 13, 40, and 134 µM). Results are expressed as the mean ± standard deviation (S.D.) of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 significantly different compared with the control group. EC, E.Cava extract; DK, Dieckol; ns, not significant; AUC, area under the curve; [Ca2+]cytol, calcium level in the cytosol.
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