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Figure 2 Evaluation of vasodilation-related protein expression. Examples of Western blot analysis, (A,E). The protein expressions of ECE treatment on (B) phosphorylated-PI3K (p-PI3K), (C) p-Akt, and (D) p-eNOS; the protein expressions of (F) p-PI3K, (G) p-Akt, and (H) p-eNOS following DK treatment. The protein bands were ultimately developed and photographed with the FUSION Solo Vilber Lourmat system. Quantitative data were analyzed using ImageJ software. Results are expressed as the mean ± standard deviation (S.D.) of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 significantly different compared with the control group. EC, E.Cava extract; DK, Dieckol; PI3K, phosphoinositide 3-kinases; eNOS, endothelial nitric oxide synthase; -: no sample treatment (control)