Figure 6

Figure 6

PPE31 promotes mycobacteria survival in macrophage and in zebrafish. (A) WT, Δppe31^Mm or comp-Δppe31^Mm-infected BMDMs were analyzed for number of surviving internalized bacilli by CFU counting at the indicated time. (B) H37Rv or Δppe31^Mtb-infected RAW264.7 cells were analyzed for number of surviving internalized bacilli by CFU counting at the indicated time. (C) Bacterial survival advantage in the infected zebrafish. Three hundred CFU of M. marinum strains were injected to reach a comparable infection level at 4 dpi. Representative burden pictures and representative burden analysis derive from larvae collected at 4 dpi. Each point in represents 1 infected larva from a representative pool. Scale bar in 200 μm. Data are shown as mean ± S.E.M. of three independent experiments. **p < 0.01. WT, wild-type M. marinum; UI, uninfected.