Effect of HBA in oxidative stress on HCD-Induced Larval Zebrafish Model in two processes. (A) The ROS production showed in fluorescence image by DCFH-DA staining and merged with a light field image. (B) The ROS (Fluorescence value) and MDA concentration of each treated larval zebrafish group; The antioxidase SOD (U/gprot) and HO-1 level (ng/ml) of each treated larval zebrafish group. Bars indicate means ± SD. n. s. indicate no significant; **p < 0.01***p < 0.001 represent compared with the control; #p < 0.05, ##p < 0.01, ###p < 0.001 represent compared with Model. p < 0.05 was considered as statistically significant calculated by One-way ANOVA followed by Tukey’s test (n = 3, n indicates the replicates of the experiment).
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