Effect of HBA in regulating lipid metabolism on an HCD-Induced Larval Zebrafish Model in two processes. (A) Experimental outline of the larval zebrafish experiment in vivo. (B) Nile red stain of larval zebrafish and fluorescent quantitation. (C) Triglyceride (TG,mmol/gprot) and total cholesterol (TC,mmol/gprot) of larval zebrafish. (D) Hematoxylin and eosin (HE) staining of larval zebrafish liver, macrovesicular steatosis, and the differences mentioned with black arrows. Bar indicate means ± SD. ***p < 0.001 represent compared with the control; #p < 0.05, ##p < 0.01, ###p < 0.001 represent compared with Model. p < 0.05 was considered as statistically significant calculated by One-way ANOVA followed by Tukey’s test (n = 3, n indicates the replicates of experiment).
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