Fig. 4

Fig. 4

a–h Control and LY294002-treated (from 32–60 hpf) embryos were fixed at 4 dpf. Markers for definitive blood lineages were assessed by in situ hybridization in the CHT: c-myb (HSPCs; a, b), globin (erythrocyte lineage; c, d), ikaros (lymphocyte lineage; e, f), l-plastin (leukocytes; g, h). Representative embryos are shown, with anterior to the left. The number of embryos that showed a particular pattern/total number of embryos is indicated in the bottom right corner of each panel. i, j GFP^high thrombocytes were imaged at 5 dpf in tg(cd41:eGFP) embryos. Scale bar: 100 µm. k–n High-resolution imaging at 12 dpf of kidney (k, l, dorsal view) (control, n = 6; LY294002-treated, n = 8; 4 µm step size) and thymus (m, n, lateral view) (control, n = 6; LY294002-treated, n = 7; 2 µm step size). Anterior to the left; maximum projections of representative larvae. Scale bar: 100 µm.