Figure 4

Figure 4

Immunohistochemical study of the olfactory rosette of zebrafish. (A,B,D,E) Anti-Calbindin labelling. (A) A sagittal section of the olfactory rosette shows labelling in a neuronal subpopulation distributed widely in the rosettes. Insets in (B) and (D) show the immunolabelling mostly located in the deeper part of the epithelium. In (B), neuroephitelial cells in the superficial layer (open arrowhead) and branches of olfactory nerves (black arrowheads) are intensely labelled. A transverse section (E) shows a similar pattern. The nonsensory epithelium (asterisk) is immunonegative. (C,F) Anti-Calretinin immunolabelling produces a stronger labelling of neuroepithelial cells, mostly concentrated in the medial part of the lamellae, and their deeper layers, whereas the nonsensory cripts (asterisk) are not immunolabelled. The neuroepithelial cells are mainly distributed in deeper layers (black arrowheads in F), and very rarely the superficial cells are lightly immunolabelled. (G–J) Anti-GFAP immunolabels isolated big cell bodies in the apical part of the epithelium. They appear in both sagittal (G) and transverse (I) sections. Insets are shown in (H) and (J), respectively. Additionally, in the olfactory bulb the antibody anti-GFAP labels the whole glomerular layer (OB). (K,L) Anti-GAP-43 immunolabelling is located in the apical part of the nonsensory epithelium (black arrowheads in K) and in individual cell bodies (black arrowheads in L) in the crypts (asterisk). (M,N) Anti-LHRH produces a light immunolabelling, mainly located in the cell processes of the nonsensory epithelium. Scale bars: 100 μm (A,C–E,G,I–M); 50 μm (B,F,H,N).