Figure 4

Figure 4

Exposure of UM cells to Cdots promotes tumorigenesis by inducing ROS. A) Images of UM cells exposed to Cdots and/or the antioxidants NAC or Toc. B) Quantitation of colony number. The addition of the antioxidants NAC or Toc reversed the Cdot‐induced procolony formation effect. The results were compared to those of the NC group. n = 3. C) Bright‐field and fluorescence images of zebrafish transplanted with UM cells labeled with CM‐Dil dye and exposed to Cdots at different concentrations (n = 5 for each group). D) Quantitation of tumor volume in fluorescence images. Cdots at 25, 50, and 100 µg mL⁻¹ promoted Mum2B cell tumorigenicity and Cdots at 25 and 50 µg mL⁻¹ promoted 92.1 cell tumorigenicity. The results were compared to those of the 0 µg mL⁻¹ group. Asterisks indicate a statistically significant difference between the control and treatment groups. n = 5, *P < 0.05. Scale bars = 100 µm.