IMAGE

FIGURE 5

ID

ZDB-IMAGE-210428-51

Genes

Antibodies

Ab2-akt

Publication

Li et al., 2021 - Calcium State-Dependent Regulation of Epithelial Cell Quiescence by Stanniocalcin 1a

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Figures for Li et al., 2021

Image

Figure Caption

FIGURE 5

Stc1a promotes NaR cell quiescence by suppressing IGF-PI3 kinase-Akt-Tor signaling in NaR cells. (A) Progenies of stc1a (+ 17)^+/–; Tg (igfbp5a: GFP) intercrosses were raised in E3 embryo medium to 3 dpf and treated with DMSO or 0.3 μM BMS-754807 (BMS). Two days later, fish were fixed and phospho-Akt positive cells in the yolk sac region were detected by immunostaining. These fish were genotyped individually afterward. n = 14–47 larvae/group. (B,C) Progeny of stc1a (+ 17)^+/–; Tg (igfbp5a: GFP) intercrosses were raised in E3 embryo medium and treated with DMSO, 0.3 μM BMS-754807 (BMS), 0.06 μM Wortmannin (Wort), 8 μM MK2206 (MK), 5 μM Rapamycin (Rapa), or 10 μM U0126 from 3 to 5 dpf. After NaR cells were quantified, these fish were genotyped individually. Representative images (B) and quantified data are shown (C). n = 7–35 larvae/group. Scale bar = 0.2 mm. (D) Progeny of stc1a (Δ18 + 1)^+/–; Tg (igfbp5a: GFP) intercrosses were raised in E3 embryo medium to 3 dpf and treated with DMSO, 0.3 μM BMS-754807 (BMS), 5 μM Rapamycin (Rapa), or 10 μM U0126 from 3 to 5 dpf. NaR cells were quantified as described above. n = 4–28 larvae/group.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol